Gallus gallus strain:GX0101 Raw sequence reads. Gallus gallus strain:GX0101

To investigate dynamic mRNA and miRNA expression profiles of Gallid herpesvirus 2 (GaHV2) -infected samples, we performed next-generation sequencing for mRNAs and miRNAs in twelve libraries, including six GaHV2-infected spleen and six mock-infected spleen at 3, 7, 14, 21, 30 and 60 dpi. A total of 16,089,905, 17,921,467, 14,921,302, 15,335,675, 24,827,231, and 16,764,369 raw reads were generated for GX0101-infected groups at the time points from 3 to 60 dpi. As for the corresponding mock groups, 19,565,208, 18,636,075, 17,509,762, 17,994,555, 18,753,333, and 15,369,671 raw reads were obtained at each time point. The sequences were blasted with chicken genome to identify its genome location. According to the criterions of fold change ≥ 2 and FDR ≤ 0.05, totals of 1,190, 1,029, 1,969, 2,782, 3,049 and 1,062 genes, listed in Table S3, were identified as DEGs between GX0101-infected and mock-infected groups at 3, 7, 14, 21, 30 and 60 dpi, respectively. Among these DEGs, 573, 606, 1,191, 1,754, 1,944 and 455 genes were observed to be up-regulated by virus infection at 3 to 60 dpi, accompanying with 617, 423, 778, 1,028, 1,105 and 607 genes down-regulated, respectively. Twelve of them were further confirmed by qRT-PCR.