4CL4 in involved in Al-resistance in rice

Aluminum toxicity is the main limiting factors of plant growth in acidic soils, aluminum ions have significant inhibitory effect of plant root growth, which affect the plant to absorb water and nutrients from soil, eventually lead to the growth of the whole plant is affected. In the process of long-term adaptation to acidic soil, rice has evolved a variety of aluminum resistant mechanisms, in small grains, rice has the strongest aluminum toxicity resistance, therefore, the study on the mechanism of aluminum resistance in rice has a positive effect on understanding the resistance mechanism of rice as well as improving the acid soil adaptability of other crops. In this study, we conducted a large-scale screening of Kasalath mutagenesis library, and obtained an aluminum resistant mutant ral1 （resistant to aluminum 1）.Aluminum accumulation is significantly reduced in root tip cell walls of ral1 compared with wild type. RAL1 encodes a 4 - Coumarate: coenzyme A ligase enzyme 4 involved in benzene propane and lignin monomer synthesis pathway. Excess p-coumaric acid and ferulic acid modified to hemicellulose prevent aluminum binding to hemicellulose.