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The PCR primers and amplicon sizes in base pairs.

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* Melting temperatures of the primers were calculated using the formula based on the nearest neighbor thermodynamic theory. The target genes of the primer position are as follows: *1: Escherichia coli 16S ribosomal RNA (Accession No. J01859) *2: Mycoplasma genitalium 16S ribosomal RNA (Accession No. NR_074611) *3: Mycoplasma hominis 16S ribosomal RNA (Accession No. NR_074603) *4: Ureaplasma parvum 16S ribosomal RNA (Accession No. NR_074762) *5: Candida albicans 18S ribosomal RNA (Accession No. AF114470) The PCR primers and amplicon sizes in base pairs.
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