Time course of brassinolide treatment on Arabidopsis seedlings

Brassinosterioids (BRs) are plant hormones known to regulate many processes such as growth and development. However, how plants respond termporally BR signaling is not as well understaood. Thus, the goal of this study was to determine the temporal transcriptional changes in Arabidopsis seedlings treated with brassinolide. We determined differentially expressed genes at each time point to see how the plant response to BR evolves over time. 7-day old seedlings were treated with brassinolide (BL) at various time points. RNA was extracted from treated and mock seedlings at each time point, then sequenced using QuantSeq.