Identification of the site of Src modification by 4-HNE using LC-MS/MS.

Sequence coverage: 70%; protein score: 28842; ion search cut off: 10. Src (10μg) was exposed to 4-HNE at a molar ratio of 1:1, digested with trypsin, and analyzed by LC MS/MS as described in Methods. Among ten ion search cut off, 4-HNE was detected in 236–260 range of amino acid. The peptide sequence containing 4-HNE adduct at amino acids, His236, Cys241, and Cys248 is denoted in bold font.a Observed values are those actually measured by LC MS/MS.b Mr(expt) are the experimental values of charge states.c Mr(calc) are relative molecular mass values calculated from the matched peptide sequence.d MASCOT scores are MS/MS ion score based on calculated probabilities.Identification of the site of Src modification by 4-HNE using LC-MS/MS.