Belowground changes to community structure alter methane-cycling dynamics in Amazonia - Meyer et al. 2020 Environment International

This is the dataset corresponding to Meyer et al. 2020. 'Belowground changes to community structure alter methane-cycling dynamics in Amazonia'. Please cite this manuscript when using any of these archived contents.<br><br>Sequences are of the V4 region of the 16S SSU rRNA gene, and were amplified using the 515F-806R primer combination.<br><br>Included here are:1) Fastq files for each sample2) Meta data file with sample names, methane flux rates, soil chemical variables, and land use types and regions (see below for further details) 'MetaDataMeyerEtAl2020_archive.txt'3) ASV community matrix of DNA-based 16S rRNA gene sequences with taxonomy assigned '16S_ASV_Table_EnvIntl_archive.txt'4) ASV IDs for known methanogens and methanotrophs 'Methanogens_trophs_EnvIntl_archive.txt'5) Community attribute table of attributes tested for relationship with methane fluxes (including qPCR data of mcrA and pmoA genes) 'AttributeTableMeyerEtAl2020_archive.txt'6) Representative sequences used to assign taxonomy to ASVs '16S_rep_dna-sequences.fasta'<br>The following Community Attribute data columns correspond to the community attributes presented in Meyer et al. 2020: 'Region' - either in the West (FNV) or the East (Santarem), 'Troph_rel_abun' - methanotroph relative abundance, 'Troph_rich'- methanotroph ASV richness, 'Gen_rel_abun'- methanogen relative abundance, 'Gen_rich' - methanogen ASV richness, 'Richness_16S' - ASV richness of the 16S-inferred community, 'Troph_prop' - proportion of methanotrophs in the methane-cycling community, 'mcra_copy_num' - normalized copy number of mcrA per g of soil, 'pmoa_copy_num' - normalized copy number of pmoA per g of soil, 'PC1-6_16S' - principle components 1 through 6 of 16S-inferred community.<br>' See publication for further details.