Transcriptomic analysis of WT, Tfam+/-, Zbp1-/- and Tfam+/-Zbp1-/- mouse embryonic fibroblasts (MEFs)

The goal of this study was to compare the gene expression profiles of WT, Tfam+/-, Zbp1-/- and Tfam+/-Zbp1-/- MEFs. Overall design: MEFs were isolated from embryonic day 14.0 embryos and passaged twice to generated enough cells for RNA seq. Total cellular RNA from MEFs was prepared using the Quick-RNA microprep kit (Zymo Research) and subjected to library preparation and RNA sequencing at the Experimental Genomics Core of the Texas A&M Institute for Genome Sciences and Society. FASTQ files were normalized, processed, and analyzed using BaseSpace Sequence Hub (Illumina). The STAR algorithm of RNAseq Alignment V2.0.0 software was utilized to align the results to the Mus musculus/mm10 (RefSeq) reference genome, then RNA-seq Differential Expression V1.0.0 software was used to obtain differential gene expression values and determine statistically significant changes in gene expression via pairwise comparison of genotypes.