Age-related transcriptional diversity in multiple skeletal muscles of mice

Aging leads to a gradual decline in muscle function, causing diseases such as sarcopenia, with different skeletal muscles responding to aging through distinct processes due to extensive transcriptional diversity. In this study, we constructed transcriptional maps of 11 skeletal muscles from young (3 month) and old (24 month) mice Female C57BL/6J mice were maintained under a 12-hour light/dark cycle with ad libitum access to standard laboratory chow. All experimental mice were of specific pathogen-free (SPF) grade. Mice were sacrificed at 3 months and 24 months of age, and 11 tissues were collected, including Ventricle (Ven), Diaphragm (Dia), Extensor Digitorum Longus (EDL), Masseter (Mas), Soleus (Sol), Gastrocnemius (Gas), Quadriceps (Qua), Tibialis Anterior (TA), Longissimus dorsi muscle (LDM), Psoas Major Muscle (PMM), and Semitendinosus (ST). The tissues were immediately flash-frozen in liquid nitrogen. We optimized the sample collection process to minimize variation.