Identification and targeting of extremely high-risk gamma delta T-ALL in children [HiChIP, ChIP and ATAC]

STAG2/LMO2 subtype in T-ALL was investigated through integrative genomic analysis. H3K27ac HiChIP was used to investigate the impact of LMO2::STAG2 translocations in MOLT-14 and PER-117 cell lines. ChIP-seq was used to explore the effects of STAG2 inactivation by using gene-edited MOLT-14 and PF382 cell lines. Overall design: Chromatin interactions in leukemia cell lines using H3K27ac HiChIP. Effects of STAG2 inactivation in gene-edited leukemia cell lines using ChIP-seq (H3K27ac, STAG1, STAG2).