Microbiome comparasion of three passerine species

In this study, we assessed the microbiome composition of three species of migrating passerines (willow warblers, lesser whitethroats and common reed warblers) captured during the spring migration stopover in Jordan in 2023. DNA extraction was carried out for a total of 59 fecal samples selected evenly from the three songbird species, and subjected to 16S microbiome analysis using Next-Generation Sequencing to determine the diversity of bacteria in fecal samples; to assess the amount of intra- and inter-specific variation in composition of the gut microbiota; and to detect pathogenic genera and species that could pose health risks to humans, domestic and wildlife animals.