First insights into the microbiome of Tunisian Hyalomma ticks gained through next-generation sequencing with a special focus on H. scupense

In this study, we have investigated the first Tunisian insight into the microbial composition of the most dominant Hyalomma species infesting Tunisian cattle and explore the relative contribution of ticks sex, life stage and species to the diversity, richness and bacterial species of tick microbiome. In this regard, next generation sequencing of the 16S rRNA (V3-V4 region) and metagenomic analysis of tick bacterial microbiota were established.