RNAseq of 2'3' cGAMP injected Drosophila melanogaster

We recently reported that an orthologue of STING regulates infection by picorna-like viruses in drosophila. Here, we show that injection of flies with 2'3'-cGAMP can induce expression of dSTING-regulated genes. Analysis of the transcriptome of 2'3'-cGAMP injected flies reveals a complex pattern of response, with early and late induced genes. Our results reveal that dSTING regulates an NF-?B -dependent antiviral program, which predates the emergence of Interferon Regulatory Factors and interferons in vertebrates. Overall design: RNAseq of Tris, ci-di-GMP and 2'3' cGAMP injected Drosophila melanogaster