HPLC and fluorometric derived phytoplankton pigment concentrations from seawater collected on BATS Validation cruises from June 1996 to June 2023

Data presented here are discrete bottle HPLC derived phytoplankton pigments and fluorometric chlorophyll-a for BATS validation (BVAL) cruises from June 1996 (BVAL cruise #50016) through June 2023 (BVAL cruise #50060). Water samples are typically collected from 7-12 depths in the upper 250 meters of the water column, and then filtered under low vacuum through a 25mm GF/F filter. The filter is then flash frozen in liquid nitrogen and stored at -80 degrees C. Shoreside, analysis is performed on an HPLC using a method modified by Dr. R. Bidigare from the Wright et al. (1991) procedure. This method identifies the pigments chlorophyll-c3, chlorophyll-c2, peridinin, 19’-butanoyloxyfucoxanthin, fucoxanthin, 19’-hexanoyloxyfucoxanthin, prasinoxanthin, diadinoxanthin, alloxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll-b, chlorophyll-a, divinyl chlorophyllide-a, alpha and beta carotene. Additionally, chlorophyll-a and phaeopigments are analyzed using a fluorometric assay.