Additional file 2 of Novel FLT3/AURK multikinase inhibitor is efficacious against sorafenib-refractory and sorafenib-resistant hepatocellular carcinoma

Additional file 2: Table S1. List of differentially expressed genes by DBPR114-treated Huh7 tumors. Tumor tissue RNA was isolated and subjected to RNA sequencing analysis using human-specific genome read data. The significantly upregulated and downregulated genes between the treatment and vehicle control groups were selected if they had an FDR < 0.05 and absolute fold change ≥ 2. n = 4 per group for vehicle control, and n = 3 per group for DBPR114. Table S2. List of down-regulated genes associated with (A) E2F targets gene set, (B) G2/M checkpoint gene set, (C) mitotic spindle gene set, and (D) MTORC complex 1 signaling gene set by DBPR114-treated Huh7 tumors. The Huh7 tumors from the vehicle control and treated groups were harvested at the end of studies (Figure 4). The tumor RNA was isolated and analyzed for human gene expressions through mRNA sequencing. Gene set enrichment analysis was performed on Huh7 xenograft tumors (FDR < 0.05).