five

Arabidopsis thaliana cultivar:Col-0 Transcriptome or Gene expression

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https://www.ncbi.nlm.nih.gov/sra/SRP051272
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Using an adapted version of nanoCAGE (nanoCAGE-2000) for sequencing on the Illumina HiSeq-2000, we sequenced the 5'' ends of gene transcripts in Arabidopsis thaliana. The protocol targets the 5'' end of transcripts by utilizing the cap-dependent template switching properties of reverse transcriptase, thereby capturing the transcriptional start sites (TSS) of RNA polymerase II genes. The end-goal being improved coverage of the TSSs while leveraging the lower RNA input requirements afforded by the nanoCAGE protocol.
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2017-11-21
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