table_1_The Prognostic Value of Immune Factors in the Tumor Microenvironment of Penile Squamous Cell Carcinoma.PDF

The host’s immune system plays a pivotal role in many tumor types, including squamous cell carcinomas (SCCs). We aim to identify immunological prognosticators for lymph node metastases (LNM) and disease-specific survival (DSS) in penile SCC. For this retrospective observational cohort study, penile SCC patients (n = 213) treated in the Netherlands Cancer Institute, were selected if sufficient formalin-fixed, paraffin-embedded tumor material was available. Analysis included previously described high-risk human papilloma virus (hrHPV) status, immunohistochemical scores for classical and non-classical human leukocyte antigen (HLA) class I, programmed death ligand-1 (PD-L1) expression, and novel data on tumor-infiltrating macrophages and cytotoxic an regulatory T-cells. Clinicopathological characteristics and extended follow-up were also included. Regression analyses investigated relationships of the immune parameters with LNM and DSS. In the total cohort, diffuse PD-L1 tumor-cell expression, CD163+ macrophage infiltration, non-classical HLA class I upregulation, and low stromal CD8+ T-cell infiltration were all associated with LNM. In the multivariable model, only tumor PD-L1 expression remained a significant predictor for LNM (odds ratio (OR) 2.8, p = 0.05). hrHPV negativity and diffuse PD-L1 tumor-cell expression were significantly associated with poor DSS and remained so upon correction for clinical parameters [hazard ratio (HR) 9.7, p

宿主免疫系统在多种肿瘤类型中扮演着至关重要的角色，包括鳞状细胞癌（SCCs）。本研究旨在识别淋巴节点转移（LNM）和特异疾病生存期（DSS）的免疫学预后指标，针对阴茎鳞状细胞癌。为此，我们进行了一项回顾性观察性队列研究，选取了荷兰癌症研究所接受治疗的213例阴茎鳞状细胞癌患者，若肿瘤材料经过足够甲醛固定和石蜡包埋，则纳入研究。分析内容包括已描述的高危人乳头瘤病毒（hrHPV）状态、经典和非经典人白细胞抗原（HLA）I类免疫组化评分、程序性死亡配体-1（PD-L1）表达、以及肿瘤浸润性巨噬细胞和细胞毒性T调节细胞的创新数据。同时纳入临床病理特征和延长随访数据。回归分析探究了免疫参数与LNM和DSS之间的关系。在总队列中，弥漫性PD-L1肿瘤细胞表达、CD163+巨噬细胞浸润、非经典HLA I类上调以及低基质CD8+ T细胞浸润均与LNM相关。在多变量模型中，只有肿瘤PD-L1表达仍然是一个显著的LNM预测因子（优势比（OR）2.8，p=0.05）。hrHPV阴性状态和弥漫性PD-L1肿瘤细胞表达与不良DSS显著相关，且在校正临床参数后仍保持相关性[风险比（HR）9.7，p