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BL26B2 experiment from the beamline training at CCP4 SPring-8 school 2018

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https://zenodo.org/record/1443391
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Raw X-ray diffraction images collected in the beamline training in day 2 of CCP4 SPring-8 school 2018 http://www.ccp4.ac.uk/schools/Japan-2018/program.php On BL26B2, strategy calculation was demonstrated using lysozyme crystals. Datasets and snapshots were collected using MX-225HS CCD detector at 1.0 and 1.5 Å wavelengths. Beam size was 120 μm (circle shape). All datasets collected in the course are available. Group1: Snapshot of cryo-cooled lysozyme crystal (well 2 of unipuck). Discussion about data collection strategy. (Max. resolution, spot overlapping, exposure time etc.) Data collection of 360 images (./data1/*.img) wavelength = 1 Å, exposure = 0.5 sec/ 0.5 deg, distance = 100 mm (edge resolution = 1.23 Å) Data processing with xdsgui. Evaluation and discussion about data quality. Group2: Snapshot of cryo-cooled lysozyme crystal (well 3 of unipuck). Discussion about data collection strategy. (Max. resolution, spot overlapping, exposure time etc.) Data collection strategy with imosflm and xdsgui, and discussion. Group3: Using snapshot of former group2, discussion about data collection strategy (especially for S-SAD) Data collection of 720 images (./data2/*.img);  wavelength = 1.5 Å, exposure = 0.5 sec/ 0.5 deg, distance = 100 mm (edge resolution = 1.85 Å) Data processing, S-SAD phasing, and model building with xdsgui.   Other entries at CCP4 SPring-8 school 2018 BL32XU https://zenodo.org/record/1442922 BL41XU https://zenodo.org/record/1443110
创建时间:
2020-01-24
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