Comprehensive analysis of circular RNA profiling in AZD9291-resistant non-small cell lung cancer cell lines

Considering the important role of circular RNAs (circRNAs) in cancers, we established AZD9291-resistant NSCLC cell lines (H1975/AZDR and HCC827/AZDR) and used microarray analysis to determine the circRNA expression profiles of the cells. The H1975/AZDR and HCC827/AZDR cell lines were induced by gradually increasing the drug concentration. CircRNA microarray expression profiles were obtained from H1975, HCC827, H1975/AZDR, and HCC827/AZDR cells and validated by quantitative reverse transcription PCR. Expression data were analyzed bioinformatically. We first established AZD9291-resistant H1975 and HCC827 cell lines (H1975/AZDR and HCC827/AZDR) by gradually increasing concentrations of AZD9291 for 72 hours with a recovery period between treatments.The newly established H1975/AZDR and HCC827/AZDR cell lines were maintained in a medium containing 10μM AZD9291, while the parental H1975 and HCC827 cell lines were cultured in drug-free medium.