Figure 2 Data

2a. Flow cytometry of bone marrow mononuclear cells (BM) and isolated monocytes (Mo). HLA-DR vs. CD11b+ is gated on live-CD45+CD3- cells, and CD14 vs. CD11b is gated on live-CD45+CD3-CD11b+ cells. 2b. Unsupervised hierarchical clustering of cytokines secreted by monocytes cultured in various matrices. Soft ~ 0.75 kPa, Stiff ~ 7.5 kPa elastic moduli. 2c. Unbiased hierarchical clustering of gene expression of monocytes in matrices from a nanoString myeloid panel. 2d. Clustering of global directed significance scores of gene set analysis. E) Principal component analysis of gene expression with donor 1 and donor 2 2f. Volcano plots of differential gene expression comparing viscous and elastic matrices of elastic modulus, E~7.5 kPa (n=3). 2g. Volcano plot of differential gene expression by nanoString in bone marrow from a cohort of patients with early (n=13) or advanced stage, overt PMF (n=16). 2h. Correlation of normalized gene expression in patients with advanced stage, overt PMF (PMF-3, n=16 biologically independent samples) with monocytes in elastic, stiff matrix (E~7.5 kPa, n=3 biological replicates from a single donor).