Hematopoietic Cell Morphology

Exhibit robustness and relevance of the combination of two image cytometry techniques: Imaging flow cytometry (IFC), and time-lapse microscopy (TLM).
Conclusion:
The strategy based on high time-resolution TLM data, obtained from a small number of cells, anchored to IFC snapshots of a large number of cells overcomes the drawbacks of both modalities. The combination of TLM and IFC shows that cell morphology reveals high fluctuations. Our approach paves the way for label-free morphological cell dynamics and offers insights for the understanding and interpretation of population data widely used in flow cytometry. Each acquisition was preceded by the assists, auto-calibration and quality control measures (on beads) included in the INSPIRE® software controlling the Image Stream X Mark II cytometer.