RNA-sequencing of dsb-2, spo-11 and htp-3 mutants to identify mechanisms involved in somatic aging of C. elegans through meiotic dysfunction

We identified that mutations in meiotic genes dsb-2, htp-3, and spo-11 accelerated the rate of aging of the organims. In order to determine mechanisms responsible for this phenotype we conducted an RNA sequencing experiment to identify potential pathways. Overall design: Comparison of adult day 1 whole worm NGS-derived transcriptomic profiles of AV157 spo-11(me44), TY4986 htp-3(y428), AV477 dsb-2(me96) animals to N2(WT) animals. For each strain, three biological replicates were collected.