Genome wide CRISPR screen for Venetoclax plus Ruxolitinib combination resistance in OCI-AML2 cells using Y. Kosuke library
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP403462
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资源简介：
30 million OCI-AML2 cells were infected with CRISPR/Cas9 library, selected for puromycin resistance for integration events and exposed DMSO, venetoclax (0.5 uM) or venetoclax (0.1 uM) plus ruxolitinib (1 uM). Cells were collected at day 14 and 21 , DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using Novaseq 6000 high throughput Illumina platform. Overall design: Performed comparisons of sgRNA read counts between DMSO and Venetoclax as well as Venetoclax plus Ruxolitinib treated cells at corresponding time points.
创建时间：
2023-09-30
