Gene expression of E.coli challenged green rice leafhopper Nephotettix cincticeps.

The green rice leafhopper Nephotettix cincticeps have two mutualistic symbiotic bacteria (Candidatus Sulcia muelleri and Candidatus Nasuia deltocephalinicola) in its symbiont special organ bacteriome and are also infected to rickettsia. In order to determine immune challenge is induced or not in N. cincticeps, we investigated gene expression of Escherichia coli challenged N. cincticeps. One day adult N. cincticeps females were pricked with E. coli with a sterile needle. Control adults were pricked with sterilized water. Total RNA of five females was extracted after 3 h and 12 h incubation respectively. E.coli challenged and control Nephotettix cincticeps samples were prepared 3 replicates for 3h and 4 replicates for 12h.