RNA-seq analysis of transcriptomic changes caused by Egg/dSETDB1 in Drosophila

Egg/dSETDB1, the ortholog of the human methyltransferase SETDB1, is the only H3K9 methyltransferase essential for viability and fertility in Drosopila. To understand the role of Egg in oogenesis, we adopted high throughput RNA sequencing (RNA-seq) and identified putative target genes regulated by Egg. Overall design: To obtain total RNAs and cDNAs, ovaries were dissected from 2- to 4-day-old wild type (w1118) or egg mutant (egg2138/Df(2R)Dll-Mp) females and frozen in liquid nitrogen.