The role of Aspergillus fumigatus SmiA transcription factor in the miltefosine resistance

By searching for new drugs against fungal pathogens, we found that miltefosine is active against Aspergillus fumigatus clinical isolates. A library of transcription factors (TF) null mutants was then challenged with this drug and we discovered a novel TF that confers resistance to miltefonise, named here SmiA. By using ChIP-seq, we searched for SmiA targets upon miltefosine treatment. Overall design: The SmiA-tagged strain was grown for 16 hours at 37oC in minimum liquid media at 220 rpm and then transferred to RPMI added with 12.5 ug/ml of miltefosine for 30 minutes (37oC 220 rpm). After crosslinking, the cells were washed and frozen for chromatin extraction and immunoprecipitation.