Data and Code: Temperature influences desiccation resistance of bumble bees

In this experiment, we crossed temperature (20, 25, and 30C) with humidity (95% RH) and measured the effects on bumble bee (Bombus impatiens) water balance. We had 10 replicates per treatment, with 6 source colonies, resulting in a total of 540 bees. We placed bees singly into tubes and checked for mortality every 2 hours for the first 12 hours and then every 4 hours until 5/10 bees from each temperature, humidity, colony treatment had reached mortality (52h and 48h for the two trials). We measured mass before and after mortality to evaluate water loss and then dried bees to a constant dry weight to get the initial water content and dry mass of each bee.

在本项实验中，我们将温度（20、25和30摄氏度）与湿度（95%相对湿度）进行交叉组合，并测量其对大黄蜂（Bombus impatiens）水分平衡的影响。每个处理组设有10个重复实验，包含6个源群，总计540只蜜蜂。我们将蜜蜂单独放入管中，并在前12小时内每2小时检查一次死亡率，之后每4小时检查一次，直至每个温度、湿度、群处理组的5/10只蜜蜂达到死亡率（两个试验分别为52小时和48小时）。在死亡率确定前后，我们测量了蜜蜂的体重，以评估水分损失，然后将其烘干至恒定干重，以获得每只蜜蜂的初始含水量和干重。