The effect of beta-glucan - induced trained immunity on the transcriptomic profile of tumor-associated neutrophils (TAN)

Induction of trained immunity by beta-glucan has been shown to promote transcriptomic alterations in myeloid cells. Neutrophils are involved in the immune response against tumors and can promote or inhibit tumor growth. In this study, we performed RNA sequencing in TAN from mice in order to investigate the impact of trained immunity on the transcriptomic profile of neutrophils in the tumor setting. WT mice were pretreated with a single dose of 1 mg of β-glucan from Trametes versicolor (Invivogen) in PBS or with PBS vehicle alone (control). After 7 days, mice were injected subcutaneously with 3 x 105 B16-F10 melanoma cells into the right flank. Neutrophils (CD45+CD11c-CD11b+Ly6c-Ly6g+) that were infiltrated in the tumor were sorted 14 days after tumor cell injection and RNA sequencing analysis was performed.