Bactericidal activities of PXDN, MPO and LPO.

E. coli K12 was incubated in 50 mM phosphate buffer (pH 6.2) containing halide anion (Cl-, Br- or I-), 10 μM H2O2, and indicated amount of hPx at 37°C for 1 h. Cell mixtures were plated on LB agar plates and incubated at 37°C overnight. The negative control experiment contained E.coli only. The CFUs were counted. The relative survival rate (%) was calculated as CFUs in the experimental group divided by those in the negative control. Data are representatives of at least three independent experiments.