Quantification of F8, VWF and AVPR2 gene expression levels in GMVECs, HUVECs and fibroblasts (relative to GMVEC levels).

RNA was extracted from untreated GMVECs, HUVECs and fibroblasts maintained in serum-free medium for 24 hours. The mRNA levels of F8, VWF, and AVPR2 were quantified relative to the mRNA levels in GMVECs after normalization to GAPDH in each PCR analysis. Data were collected from 4–7 separate RNA extractions using each cell type.aIndicates down-fold expression levels; 0.64 = 1.5 down-fold, 0.20 = 5 down-fold, and 5.1 (10−5) = 19,607 down-fold.Quantification of F8, VWF and AVPR2 gene expression levels in GMVECs, HUVECs and fibroblasts (relative to GMVEC levels).