Gene expression profiles of newly generated MLL-AF9 leukemia cells and aggressive MLL-AF9 leukemia cells generated through serial transplantations.

Immunogenicity of acute myeloid leukemia (AML) keeps changing during tumor evolution, showing a trend that the aggressive AMLs generate through serial transplantations are susceptible to NK cell-mediated tumor suppression. To access molecular differences between the newly developed AML cells and aggressive AML cells generated through serial transplantations, we performed RNA-seq using newly generated mouse AML cells trasduced with MLL-AF9, and the aggressive MLL-AF9 cells obtained after 5 times serial transplantations in the non-irradiated recipient mice. Overall design: Newly generated or aggressive MLL-AF9 leukemia cells coexpressing GFP were transplanted into C57BL/6 mice. GFP+ leukemia cells were collected from bone marrow and isolated using cell sorter before RNA extraction.