佳味二芝丸通过调节 SIRT1/JNK/p38 MAPK 通路改善雄激素性脱发

雄激素性脱发 （AGA） 是一种脱发。我们之前的研究表明，AGA 改善草药处方“甲味二之碗”（WJWE） 的水提取物具有 AGA 改善能力，该处方源自传统配方“二之碗”。然而，其潜在机制仍然未知。本研究采用 UPLC\u2012MS/MS 分析对 WJWE 中的植物化学成分进行表征。采用二氢睾酮 （DHT） 诱导的小鼠模型和真皮细胞 （DPC） 测定来评价和阐明 WJWE 对 AGA 的有益影响和机制。WJWE 通过刺激 Wnt5A/β-Catenin 通路，促进 AGA 小鼠的毛发生长和毛囊再生，改善 DPCs 生长和剂量依赖性保护的 DHT 减少的 DPCs 在体外的活力 <i>in vitro</i> 。此外，WJWE 减少了 AGA 模型小鼠皮肤和 DHT 处理的 DPC 中 DHT 诱导的氧化应激。为了阐明调节机制，我们发现 WJWE 处理在 DHT 处理的 DPCs 和 AGA 模型小鼠中显着且剂量依赖性地增加了 SIRT1 的表达并降低了 JNK 和 p38 MAPK 的磷酸化。而 EX527 （一种 SIRT1 抑制剂） 的应用可以起 WJWE 的作用。我们的研究提供了 WJWE 治疗 AGA 的一些证据，其中 SIRT1/JNK/p38 MAPK 信号通路可能是主要靶点。

Androgenetic alopecia (AGA) is a type of hair loss. Our previous study demonstrated that the aqueous extract of Jiawei Erzhi Wan (WJWE), an AGA-improving herbal prescription derived from the traditional formula Erzhi Wan, exhibited potential AGA-relieving effects. However, its underlying therapeutic mechanism remains largely unclear. In this study, UPLC–MS/MS analysis was employed to characterize the phytochemical components present in WJWE. A dihydrotestosterone (DHT)-induced mouse model and dermal papilla cells (DPCs) assay were utilized to evaluate and elaborate the beneficial effects and underlying mechanism of WJWE against AGA. WJWE promoted hair growth and follicular regeneration in AGA mice by activating the Wnt5A/β-Catenin signaling pathway, enhanced the proliferation of DPCs, and dose-dependently protected the viability of DHT-reduced DPCs in vitro. Furthermore, WJWE alleviated DHT-induced oxidative stress in the skin of AGA model mice and DHT-treated DPCs. To elucidate the specific regulatory mechanism, we found that WJWE treatment significantly and dose-dependently upregulated the expression of SIRT1 and downregulated the phosphorylation of JNK and p38 MAPK in both DHT-treated DPCs and AGA model mice. Notably, the application of EX527, a selective SIRT1 inhibitor, could exert the same effects as WJWE. Our study provides credible evidence supporting the therapeutic potential of WJWE for AGA, with the SIRT1/JNK/p38 MAPK signaling pathway likely serving as the core therapeutic target.

搜集汇总

背景与挑战

背景概述

该数据集研究了中药'佳味二芝丸'对雄激素性脱发的治疗机制，发现其通过调节SIRT1/JNK/p38 MAPK通路促进毛发生长和毛囊再生，并在小鼠模型和细胞实验中证实了剂量依赖性保护作用。