Immunological responses to tetanus and influenza vaccination in donkeys

The dataset concerns the immunological response of donkeys (Equus asinus) to a combined vaccination against tetanus and equine influenza. The data were collected in an experimental study aimed at assessing temporal changes in T and B lymphocyte populations as well as monocyte subpopulations using flow cytometry. This dataset enables evaluation of cell-mediated immune responses in donkeys—a species for which such data are extremely limited in the literature—making it a unique contribution to the fields of veterinary and comparative immunology.The dataset consists of raw tabular result summaries (xlsx, ods). These files document the percentage and mean fluorescence intensity (MFI) of selected immune cell subsets (e.g., CD4+, CD8+, FoxP3+, CD5+, PanB, CD14+) at three time points: pre-vaccination, 30 days after the initial dose, and 30 days after the booster dose. The .csv files also contain demographic information (age, sex) and metadata related to sample collection (date, individual ID).Data acquisition was performed using a BD FACSCanto II flow cytometer and analyzed with Kaluza 1.5 software. Peripheral blood mononuclear cells (PBMCs) were isolated using SepMate™–Lymphoprep™ kits. In vitro stimulation was carried out using a PMA/ionomycin cocktail with protein transport inhibitors. Antibodies used in this study were selected based on confirmed cross-reactivity with Equus asinus antigens.