analysis of the microbiota of black stain in the primary dentition. human oral metagenome

Black tooth stain is a characteristic extrinsic discoloration commonly seen on the cervicalenamel following the contour of the gingiva. To investigate the relationship between blacktooth stain and the oral microbiota, we used 16S rRNA gene sequencing to compare themicrobial composition of dental plaque and saliva among caries-free children with and withoutblack stain.