Sequencing_the_Microbiome_of_the_Jumbo_mouse__an_infection_model_for_otitis_media

Animal models offer a critical opportunity to investigate the mechanisms that underlie the switch from commensal to disease-causing behaviour in the upper respiratory tract that leads to disease. In vivo studies of OM are increasingly focussed on the use of inbred strains of mice and mutant mouse lines, these can overcome deficiencies associated with the chinchilla (Chinchilla lanigera), a frequently used polymicrobial model of OM. We have developed a model with NTHi for ME infection in the Junbo mouse, a mutant mouse line that spontaneously develops chronic ME inflammation under specific pathogen free (SPF) conditions.The Microbiome will be analysed by direct sequencing of rDNA in mouse samples (16s RNA) and if required by high throughput genome sequencing following isolation and limited culture of samples