Single-Cell Transcriptomic Analysis Reveals Neutrophil as Orchestrator during ß-Glucan-Induced Trained Immunity in a Teleost Fish

We establish a trained immunity activation model in turbot (Scophthalmus maximus) by training with ß-glucan in vivo. Through single cell RNA-sequencing analysis, we annotate 16 clusters of immune cells and blood cells from head kidney and spleen, and successfully characterize that neutrophils exhibit distinguished feature of trained immunity. Overall design: Single cell transcriptome analysis was performed to study the feature of ß-Glucan induced trained immunity in a teleost fish