LC-C202508150042

In this study, we have uploaded the transcriptome sequencing dataset of GL3 leaf explants following callus induction culture with sodium butyrate to the NCBI database.The detailed experimental treatments and culture conditions are as follows: GL3 leaves were used as experimental materials and treated with 0.1 micromol/L sodium butyrate (NaB, a histone deacetylase inhibitor). The treated GL3 leaves were processed into explants by longitudinally cutting perpendicular to the leaf veins, removing leaf tips and petioles, and retaining leaf segments with incisions at both ends. These leaf segment explants were then inoculated onto a callus induction medium supplemented with a histone deacetylase inhibitor, cultured in the dark for 20 days and 30 days, respectively, and the harvested materials were subjected to transcriptome sequencing.This dataset documents transcript expression profiles of GL3 leaf explants at distinct time points during sodium butyrate-induced callus formation. It enables dissection of the molecular regulatory network by which deacetylation modifications govern callus initiation from plant leaf explants, facilitates identification of key genes associated with callus development, and provides foundational transcriptomic data for investigating the molecular mechanisms of plant somatic embryogenesis and tissue culture regeneration. The dataset has been successfully uploaded to the NCBI database and is available for query and secondary analysis by researchers in plant molecular biology and plant tissue culture.