CUSP9-9096

Exhaustive in vitro evaluation of the 9-drug cocktail CUSP9 for treatment of glioblastoma using COMBImageDL. Label-free live-cell imaging data for the second replicate 384-well plate (barcode 9096) containing all plausible drug combinations of order 5-9 from the CUSP9 protocol as add-on to standard-of-care Temozolomide, using the human glioblastoma cell line M059K. 22 grayscale images/frames of size 800x992 were acquired per experimental well, one every 4h for 84h, starting without treatment at 0h. The first and second channel of each frame contain the original grayscale pixel values, while the third one contains the binary segmentation map (foreground vs. background) generated with a modified version of the deep convolutional neural network architecture U-Net. M059K_190429_9096.xlsx: Excel file containing the experimental layout designed for plate 9096 and used to produce the corresponding transfer scheme for acoustic liquid dispension.采用COMBImageDL对用于治疗胶质母细胞瘤的九药联合方案CUSP9开展全面体外评价。本数据集包含采用人类胶质母细胞瘤细胞系M059K开展的实验数据：以临床标准治疗方案替莫唑胺（Temozolomide）为基础疗法，添加CUSP9方案中5至9阶所有合理药物联合组合，对应第二重复384孔板（条形码9096）的无标记活细胞成像数据。每个实验孔均采集尺寸为800×992的灰度图像/帧共22张，于0小时（未施加任何处理）开始，每4小时采集1张，持续时长84小时。每帧图像的第一、第二通道存储原始灰度像素值，第三通道则存储经改进版深度卷积神经网络架构U-Net生成的二值分割图（区分前景与背景）。M059K_190429_9096.xlsx：该Excel文件包含针对9096号孔板设计的实验布局，用于生成对应的声学液体分配方案。

搜集汇总

背景与挑战

背景概述

该数据集包含CUSP9九种药物组合联合替莫唑胺治疗胶质母细胞瘤的活细胞成像数据，使用384孔板，每孔采集22个时间点的灰度图像（共84小时），并附带由U-Net生成的二值细胞分割图，用于评估药物组合疗效。