BCG vaccination stimulates Integrated Organ Immunity by feedback of the adaptive immune response to imprint prolonged innate antiviral resistance

Bacille Calmette-Guérin (BCG) vaccination can confer non-specific protection against heterologous pathogens. However, the underlying mechanisms remain mysterious. We used scRNA-seq in this study to identify transcriptional changes in the immune and epithelial cells in the lungs following BCG vaccination in mice Overall design: Lung immune and epithelial cells were enriched by Fluorescence-activated cell sorting using respective cell markers and mixed with non-enriched fraction for scRNA-seq