GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration

The saltatory migration of neurons is essential for brain formation. Whether mechanical stimuli regulate this process is unknown. Here we show that the primary cilium acts as a mechanical sensor through GPR161. Using an ex vivo neuronal migration model and microfluidic assays, we demonstrate that fluid shear stress induces migration via the mechanoreceptor GPR161 at the primary cilium, with its mechanosensitive Helix 8 being essential. We demonstrate that GPR161 activates a recently discovered cAMP/PKA signaling pathway leading to the phosphorylation of NDE1, a dynein complex regulator, and microtubule organization to regulate migration. These findings unveil a dynamic primary cilium-based pathway sensing mechanical stimulus to drive cyclic saltatory neuronal migration during brain development., , , # GPR161 mechanosensitivity at the primary cilium drives neuronal

saltatory migration†: Open Source Data

[https://doi.org/10.5061/dryad.zkh1893mz](https://doi.org/10.5061/dryad.zkh1893mz)

Other publicly accessible locations of the data:
[https://dropsu.sorbonne-universite.fr/s/aBwfsN7F2sFt9Q8](https://dropsu.sorbonne-universite.fr/s/aBwfsN7F2sFt9Q8)

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We have submitted our raw data for the paper \"GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration\".

This includes the raw data:

\- Fig. 1D

\- Fig. 1F

\- Fig. 1G

\- Fig. 1HIJ

\- Fig. 2C

\- Fig. 2D

\- Fig. 2E

\- Fig. 2F

\- Fig. 3C

\- Fig. 3D

\- Fig. 3G

\- Fig. 4D

\- Fig. 4EFG

\- Fig. S2D

\- Fig. S3B

\- Fig. S4

## Description of the data and file structure

Each file contains a simple Excel file of the raw data of quantified parameters.

### Title: Fig. 1D

Excel table presenting the number of GFP+ RMS neuroblasts presenting a GPR161+/Arl13b+ or GPR161-/Arl13b+ immunoreactive PC in miRN...,