Dataset for comparative phenotypic and genotypic profiling of ESBL- and carbapenemase-associated Gram-negative bovine mastitis pathogens in western Türkiye, 2025–2026

This dataset supports the manuscript entitled “Comparative phenotypic and genotypic profiling of ESBL- and carbapenemase-associated Gram-negative bovine mastitis pathogens in western Türkiye, 2025–2026.” The dataset includes anonymized data for 160 non-duplicate Gram-negative bovine mastitis isolates recovered from quarter-milk samples collected from dairy farms in western Türkiye between May 2025 and January 2026. The included isolates comprise Escherichia coli, Klebsiella pneumoniae, and Acinetobacter baumannii, classified into ESBL-producing E. coli, carbapenem-resistant E. coli, ESBL-producing K. pneumoniae, carbapenem-resistant K. pneumoniae, and carbapenem-resistant A. baumannii groups. The Excel file contains isolate metadata, mastitis origin, antimicrobial susceptibility testing results, resistance-group classification, ESBL phenotypic confirmation, carbapenemase assay results, PCR detection of beta-lactamase genes, MDR/XDR classification, statistical summaries, and figure/source-data tables used in the manuscript. Farm and animal identifiers were anonymized before deposition. The dataset is intended to support transparency, reproducibility, and reuse of the phenotypic, molecular, and statistical analyses reported in the associated manuscript. The description matches your manuscript design: 450 quarter-milk samples from 504 cows and 15 farms, yielding 160 non-duplicate Gram-negative isolates, including 60 E. coli, 68 K. pneumoniae, and 32 A. baumannii.