Bulk RNA-seq and ATAC-seq of mouse uterine tissue-resident natural killer cells.

Uterine natural killer(NK) cells play a crucial role in pregnancy by promoting spiral artery remodeling and secreting fetal growth factors.Uterine trNK cells before pregnancy and during pregnancy (gd6.5, gd8.5 and gd11.5) were sorted, then applied into bulk RNA-sequence to reveal the features at different timepoints. Similarily, uterine trNK cells with different treatment in vitro were also sorted for bulk RNA-sequence to identify the role of IL-21-STAT3 signaling. To interpret the differentiation trajectory of uterine trNK cells, CD45+NKp46+NK1.1+ cells in wild-type virgin mice, wild-type gd8.5 mice, Il21r-/- gd8.5 mice and Il21r-/- gd8.5 mice with Caspase3 inhibitor are sorted.Applying them to Single-Cell RNA sequence, a differentiation trait of uterine NK cells during pregancy was revealed and IL-21 significantly promoted this process at initial stage. We aimed to reveal the differentiation trajectory of uterine natural killer cell during pregnancy and how IL-21 promote this process. To investigate the regulated mechanism of uterine trNK differentiation, uterine trNK were treated with IL-21 in vitro. At different time cells were collected and sorted for bulk RNA-sequence and ATAC-seq.