Deep 16s rRNA pyrosequencing reveals a bacterial community associated with petroleum hydrocarbon fractions degradation by PGPR enhanced phytoremediation. petroleum phytoremediation

Background and aims This study aimed to investigate petroleum phytoremediation enhancement by plant growth promoting bacteria (PGPR), specifically the correlation between petroleum hydrocarbons fractions and bacterial community structure affected by remediation and PGPR inocula.Aged petroleum contaminated soil was remediated by tall fescue (Testuca arundinacea L.) inoculated with two PGPR strains respectively. Hydrocarbon degradation was measured by GC-MS (Gas-chromatography Mass-spectrometer) based on carbon fraction numbers (C8-C34) and bacterial community structure change has been detected based on 16s rRNA by high-throughput pyrosequencing.