Expression data from breast cancer cell line 4T1

MiR-9 plays an irreplaceable role in regulating the progression of breast cancer. In order to study the role of miR-9 in promoting MDSC recruitment in the tumor microenvironment, we need to explore the genetic process of miR-9 regulation. We used microarrays to detail the global program of miR-9 down-regulated gene expression and identified different categories of regulated genes in this process. A breast cancer cell line with stable knockdown of miR-9 was constructed by lentivirus infection for RNA extraction and Affymetrix microarray hybridization. The experimental group and the control group were repeated 3 times.