ChIP-seq analysis of replicating Merkel Cell Polyomavirus genomes. MCPyV ChIP-seq

Chromatin was immunoprecipitated from PFSK1 cells transfected with either wild type Merkel Cell Polyomavirus (MCVSyn) or a viral mutant harboring mutations in the region upstream of the genomic locus of the viral miRNA mcv-miR-M1 (MCVSyn-pmt). Chromatin immunoprecipitations were carried out with antibodies directed against the MCPyV LT-Antigen (LT-Ag), H3 histones trimethylated at lysine 4 (H3K4me3), or with an anti-IgG antibody control. ChIP-seq reads were mapped against the parental genomes. The start position of the reference sequences was moved to nucleotide position 3119 (relative to GenBank accession JN707599, MCVSyn) to facilitate mapping of reads at the viral origin of replication.