Microbiome characterization using transfer RNA sequencing

Here we report a direct tRNA sequencing protocol and software to simultaneously examine the composition and biological activity of naturally occurring microbial communities. Our analysis of mouse gut microbiome with tRNA-seq and 16S ribosomal RNA gene amplicons revealed comparable microbial community structures, and additional physiological insights into the microbiome through tRNA abundance and modifications. Comprehensive analysis of high throughput sequencing data to determine microbial community structures in mouse gut microbiome (tRNA profiling dataset).