Fig5d Sperm staining of caudal epididymal sperms.

Raw image dataset for sperm staining of caudal epididymal sperms from Wdr62 genetrap and control mice. <br>An incision was made to the caudal end of isolated epididymus in DMEM (Gibco). This was followed by 15 min incubation at 37°C to allow spermatozoa to swim out. Sperms were centrifugated at 150g for 5 min to remove DMEM. Sperms were fixed in 95% ethanol for 15 min and 10μL was smeared onto a glass slide and air-dried for 2 hrs. Slides were kept at 4°C overnight. The next day, sperm stain was performed using the Papanicolaou method as described in https://doi.org/10.1093/eshremonographs/2002.2.13, with 3 min incubation in Harris hematoxylin (Sigma), 2 min in orange G6 (OG6 0.5% solution in 95% ethanol, phosphotungstic acid 0.015g/100ml) and 5 min in EA50 (0.5% lightgreen SF yellowish CI 42095, 0.5% bismark brown CI 21000, 0.5% eosin Y CI 45380, 0.2g phosphotungstic acid and 2 drops of lithium carbonate (0.154g in 10ml dH₂O)). <br>