Rescue Expression of dSarm1 transgenes rescue lethality of dSarm1 mutants.

Daughterless-GAL4 (da-GAL4) was used to drive ubiquitous expression of Sarm1 transgenes. Homozygous dSarm14705/dSarm14621 mutant animals are lethal. Expression of transgenes in the mutant background tests for the ability of the transgene to rescue dSarm1 function. Expression of transgenes in a WT background tests for lethality of the transgene itself as is seen in the case of dSarm1CE. Quantification is shown for each genotype as two sets of numbers 1) number of rescued mutant flies relative to control flies and 2) WT flies expressing transgene versus control flies). Quantification is as follows: da-GAL4/+ (0 mutant+transgene: 220 control and 144 WT+transgene: 66 control); da-GAL4/ dSarm1 (3KB) (166:73 and 171:159); da-GAL4/dSarm1ED (3KB) (0:239 and 191:182); dSarm1CE (3KB) (0:251 and 0:262); da-GAL4/dSarm1CE+ED (3KB) (0:201 and 225:185); da-GAL4/dSarm1 (5KB) (204:63 and 90:243); da-GAL4/dSarm1ED (5KB) (0:187 and 199:90); da-GAL4/dSarm1CE (5KB) (0:164 and 0:195); da-GAL4/dSarm1CE+ED (5KB) (0,153 and 185:206); da-GAL4/human SARM1 (0,370 and 288:196).