Analytical Comparison of Illumina and Oxford Nanopore Sequencing for Drug Resistance Detection in Mycobacterium tuberculosis: A Cross-Sectional Study from Antioquia, Colombia

The objective of this study was to evaluate the effectiveness of Oxford Nanopore Technologies (ONT) in comparison to Illumina sequencing for the identification of genetic variants associated with resistance to first- and second-line anti-tuberculosis drugs. This approach was assessed for its reliability, accuracy, and practical applicability as a potential alternative to conventional short-read sequencing platforms, particularly in resource-limited settings.