DataSheet_3_Prevalence and antibiotic resistance of Staphylococcus aureus associated with a college-aged cohort: life-style factors that contribute to nasal carriage.pdf

Staphylococcus aureus is an opportunistic human pathogen that can frequently be found at various body locations, such as the upper respiratory tract, nostrils, skin, and perineum. S. aureus is responsible for causing a variety of conditions, which range from minor skin infections and food poisoning to life-threatening sepsis and endocarditis. Furthermore, S. aureus has developed resistance to numerous antimicrobial agents, which has made treatment of S. aureus infections difficult. In the present study, we examined lifestyle factors that could increase the likelihood of S. aureus carriage, the overall prevalence of S. aureus, as well as assessed the antibiotic resistance profiles of the S. aureus isolates among a population of college students. Five hundred nasal samples were collected and analyzed via selective growth media, coagulase and protein A testing, as well as polymerase chain reaction and DNA sequencing. One hundred four out of the 500 samples collected (21%) were identified as containing S. aureus. The S. aureus isolates were resistant to penicillin (74%), azithromycin (34%), cefoxitin (5%), ciprofloxacin (5%), tetracycline (4%), and trimethoprim (1%), but sensitive to gentamicin and rifampin. Lastly, we identified several lifestyle factors (i.e., pet exposure, time spent at the university recreational facility, musical instrument usage, and tobacco usage) positively correlated with S. aureus nasal colonization.

金黄色葡萄球菌是一种机会性的人类病原体，常存在于人体的多个部位，如上呼吸道、鼻孔、皮肤和会阴部。金黄色葡萄球菌可引起多种疾病，其严重程度从轻微的皮肤感染和食物中毒到危及生命的败血症和心内膜炎不等。此外，金黄色葡萄球菌对多种抗菌药物产生了耐药性，这使得金黄色葡萄球菌感染的治疗变得尤为困难。在本研究中，我们探讨了可能增加金黄色葡萄球菌携带可能性的生活方式因素，评估了金黄色葡萄球菌在大学生群体中的总体流行率，以及分析了金黄色葡萄球菌分离株的抗生素耐药谱。共收集并分析了500份鼻拭子样本，通过选择性培养基、凝固酶和蛋白A检测、聚合酶链反应和DNA测序等方法进行。在收集的500份样本中，有104份（21%）被鉴定为含有金黄色葡萄球菌。金黄色葡萄球菌分离株对青霉素（74%）、阿奇霉素（34%）、头孢西丁（5%）、环丙沙星（5%）、四环素（4%）和甲氧苄啶（1%）具有耐药性，但对庆大霉素和利福平敏感。最后，我们还发现了一些与金黄色葡萄球菌鼻部定植呈正相关的生活方式因素，例如宠物接触、在大学娱乐设施中的逗留时间、乐器使用和烟草使用。