Transcriptomic analysis of the iron depletion in yeast

The goal of the project was to study the effects on transctiption and mRNA stability after iron depletion. We analyzed the effect of iron depletion caused by 100 μM of the Fe2+-specific chelator bathophenanthroline disulfonic acid (BPS) on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 0, 10, 30, 90, 180 and 360 minutes after iron limitation. Cells were grown in YPD at 30ºC to 0.2 OD600. At the indicated times after BPS addition cells were spun-down and collected. Please note that each sample record represents three replicates (more details in the sample description field).